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Family Farm Seaside Full Version. Last Updated 2019-09-25 11:09.. of mice and the amount of glucose in the blood. This was used to calculate the blood glucose concentration (mmol/L).
d)Respiratory rate: Animals were anaesthetized with a combination of ketamine (Imalgene 1000, Merial) and medetomidine (Domitor, Orion Pharma), the carotid artery was isolated, a blood sample was taken and the animals were killed by cervical dislocation. Immediately after death, the animals were placed on a warm plate, the thorax was opened and the trachea was cannulated to facilitate respiration. The respiratory rate of the animals was measured as the number of times the chest wall was lifted during a 30 s observation period and recorded as the number of counts per minute.
The lower and upper limit for each of these measurements are given in Table 1. The results are presented as means ± SEM.
Behavioural tests
Table 2 shows the behavioural tests used in this study.
Behavioural tests:
Table 2.
Table 2.
Evaluation of the cognitive-deficits in mice subjected to long-term prenatal ethanol exposure.
Measurement
Test
Operant conditioning
Percentage of pellets obtained from the apparatus during five consecutive days of training
Open field test
Total distance travelled in the maze
Morris water maze
Total distance travelled and swimming speed in the target quadrant
Elevated plus maze
Total distance travelled and the number of arm entries
Social recognition
Percentage of time spent in the social arena
Conditioned taste aversion
Percentage of ethanol-saline consumption
The mice were allowed to adapt to the behavioural test apparatus for a period of five days prior to testing, in order to control for non-specific effects of the testing.
The maternal drinking paradigm
Maternal drinking: The method for monitoring ethanol consumption by the dams is based on the previous work from our laboratory (Akerström et al., 1996). In short, dams were housed singly and given access to ethanol 3 d/week for a period of 4 weeks (EtOH group). In the ethanol-consuming periods, 0b46394aab
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